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为了充分利用河资源 ,开展了河安全利用课题研究。利用高效液相色谱法和竞争抑制性酶联免疫吸附试验 (ELISA)方法对安全利用过程中的河毒素进行检测 ,同时引入危害分析和关键控制点 (HACCP)系统 ,对河食品从原料生产到成品的各环节进行危害分析 ,确定关键控制点。制订了原料接收、活鱼暂养、加工去毒、河制作、以及环境卫生的控制标准和措施。结果表明 ,河的分布具有地域差异性。河体内毒素分布很不均匀 ,主要集中在肝、卵巢、皮肤等部位 ,而且季节性变化明显 ,个体差异显著。运用该研究成果自 1995年至 2 0 0 2年进行人体试食试验以来 ,未发现一例中毒症状和体征 ,各项试验证明 ,经去毒工艺处理的河是安全的 ,经食用后不会引起河毒素中毒。这对于保证河资源的安全利用有着重大的意义。
In order to make full use of the resources of Hejian, the study on the safety utilization of Hejian has been carried out. High-performance liquid chromatography (HPLC) and competitive inhibition enzyme-linked immunosorbent assay (ELISA) were used to detect the tetrodotoxin in the process of safe utilization. At the same time, the system of hazard analysis and critical control point (HACCP) Production to the finished product of all aspects of hazard analysis to determine the key control points. Formulated a raw material acceptance, live fish holding, detoxification, river production, and environmental sanitation control standards and measures. The results show that the distribution of the river has geographical differences. River toxin body toxins distribution is very uneven, mainly in the liver, ovary, skin and other parts, but seasonal changes significantly, the individual significant difference. Using the results of this study from 1995 to 2002, human feeding test since the discovery of a case of poisoning symptoms and signs, the test proved that detoxification process of the river is safe, after eating will not Rehmannia toxins poisoning caused. This is of great significance for ensuring the safe utilization of resources in rivers and lakes.