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在妊娠期间胎儿细胞进入母体循环系统,这可为产前诊断提供一种安全、可靠的非侵入性测验手段。不过,合适的细胞类型的选择和富集的方法是需要优先考虑的重要问题。多数研究者认为选择胎儿的有核红细胞(NRBCs)较为恰当,因为它们属于单个核细胞,胎儿血中的数量充盈,相对地分化较好而且生命期限较短。在40例妊娠妇女怀孕16到18周期间收集外周血样品20ml。富集NRBCs,作者采用了阴性磁性细胞分离(MACs),
Fetal cells enter the maternal circulatory system during pregnancy, which provides a safe, reliable and noninvasive test for prenatal diagnosis. However, the selection and enrichment of suitable cell types is an important issue that needs to be prioritized. Most researchers believe that selecting fetal nucleated erythrocytes (NRBCs) is more appropriate because they belong to mononuclear cells, which are plentiful, relatively well differentiated, and have a short lifespan. Peripheral blood samples 20 ml were collected during the 16 to 18 weeks of gestation in 40 pregnant women. Enriching NRBCs, the authors used negative magnetic cell isolation (MACs)