论文部分内容阅读
为对我国东北地区分离的斑点热群立克次体分离株作鉴定,建立并应用了分子立克次体学方法,包括单克隆抗体、SDS-PAGE、免疫印迹、DNA 酶切图谱、PCR/RFLP、PCR/SSCP 及 DNA 序列分析等。结果表明:①在种的水平上研究了黑龙江分离株(代表株 HLJ-54、36),从分类学上确定其属立克次体属、斑点热群的一个新种,命名为黑龙江立克次体(R.heilongjiangii);②发现从疑似病人血液分离的 H-5株的分子生物学特征与黑龙江立克次体一致,证实了其对人的致病性。
In order to identify the spotted heat group Rickettsia isolates isolated in Northeast China, molecular rickettsial method was established and applied, including monoclonal antibody, SDS-PAGE, western blot, restriction enzyme digestion, PCR / RFLP, PCR / SSCP and DNA sequence analysis. The results showed that: (1) Heilongjiang isolate (representative strain HLJ-54,36) was studied at species level, and a new species belonging to genus Rickettsia (R.heilongjiangii). ② The molecular biological characteristics of H-5 isolates isolated from the blood of suspected patients were found to be consistent with those of Rickettsia in Heilongjiang Province, confirming their pathogenicity to humans.