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从华南沿海各病区采集的6个木麻黄青枯菌分离株,通过对寄主范围、浸润反应和色素形成的测定,均表现能侵染多种茄科植物,而不能侵染香蕉;浸润于烟叶上引致坏死反应;在含L—酪氮酸的培养基上产生大量黑素。因此,确认其属于青枯荫小种1。各分离株均能利用半乳糖醇、山梨糖醇和甘露糖醇产酸,而对乳糖、麦芽糖和纤维二糖的利用能力则不同,分属于Hayward的生化型Ⅲ和Ⅵ。各分离株的其它细菌学特性也稍有差异。根据各分离株的致病性、菌落形态、生化反应和血清反应的差异,上述6个木麻黄青桔菌分离株可划分为三个菌系,SC—1、SC—2和SC—3。
Six R. solanacearum isolates collected from different wards in the coastal areas of South China showed that they could infect many solanaceous plants and could not infect bananas through the determination of host range, infiltration reaction and pigment formation. Tobacco leaves cause necrosis reaction; L-tyrosine containing medium to produce a large number of melanin. Therefore, it is confirmed that it belongs to the green shade seed 1. All isolates were able to utilize acid from galactitol, sorbitol and mannitol, while their ability to utilize lactose, maltose and cellobiose was different, belonging to Hayward’s biochemical class III and VI. Other bacterial characteristics of each isolate are also slightly different. According to the differences of pathogenicity, colony morphology, biochemical reaction and serum reaction of the isolates, the six isolates of M. avium were divided into three strains, SC-1, SC-2 and SC-3.