论文部分内容阅读
本实验采用华西医科大学第一附属医院门诊１９９２年上半年获取的５５例鼻咽部低分化鳞癌（ＮＰＣ）的活检存档标本，用１５例正常人鼻咽部组织蜡块作对照。所有标本作免疫组化ＬＳＡＢ法染色，测定Ｐ蛋白的表达。并对所有ＮＰＣ患者进行５年随访。结果表明，Ｐ蛋白在鼻咽部正常组织表达阳性率为１００％，鼻咽部低分化鳞癌阳性率为７０．９％，二者差别有统计学意义（Ｘ２＝４．１３，Ｐ＝０．０４７２）。Ⅰ－Ⅱ期、Ⅲ期和Ⅳ期ＮＰＣＰ蛋白表达阳性率分别为８３．３％、８０．０％、６０．７％，差别有统计学意义（Ｋｗ＝８．３０５０，Ｐ＝０．０４７３）；Ｔ１－Ｔ２、Ｔ３和Ｔ４期ＮＰＣ的Ｐ蛋白表达阳性率分别为８０．８％、７２．２％、４５．５％，差别有统计学意义（Ｋｗ＝１５．３４，Ｐ＝０．０３９４）；无颈淋巴结转移与有颈淋巴结转移组Ｐ蛋白表达阳性率分别为７７．８％、７０．３％，差别无统计学意义（Ｋｗ＝６．２７９３，Ｐ＝０．０５５７）；Ｐ表达阴性组与阳性组的５年生存率分别为：５６．７％、６８．１％，二者差别无统计学意义（Ｐｒ＝０．４７７５）。通过本实验提示：在ＮＰＣ的发生发展过程中有Ｐ１５蛋白丢失的参与；Ｐ１５蛋白表达可作为ＮＰＣ生物学行为的一个参考指标?
Fifty-five biopsied specimens of nasopharyngeal poorly differentiated squamous cell carcinoma (NPC) were obtained from the First Affiliated Hospital of Huaxi Medical University in the first half of 1992, and the control samples were obtained from 15 normal human nasopharyngeal tissues. All specimens were stained with immunohistochemical LSAB method to determine the expression of P protein. All NPC patients were followed up for 5 years. The results showed that the positive expression rate of P protein in normal nasopharyngeal tissues was 100%, and the positive rate of poorly differentiated nasopharyngeal squamous cell carcinoma was 70.9%. The difference was statistically significant (X2 = 4.13, P = 0 .0472). The positive rates of NPCP protein in stageⅠ-Ⅱ, stageⅢandⅣwere 83.3%, 80.0% and 60.7%, respectively, with statistical significance (Kw = 8.3050, P = 0.0473) ; The positive rates of P protein in T1-T2, T3 and T4 NPC were 80.8%, 72.2% and 45.5%, respectively, with significant difference (Kw = 15.34, P = 0.0394 ). The positive rate of P protein in non-cervical lymph node metastasis and cervical lymph node metastasis was 77.8% and 70.3%, respectively, with no significant difference (Kw = 6.2793, P = 0.0557) The 5-year survival rates of the negative group and the positive group were 56.7% and 68.1% respectively, with no significant difference (Pr = 0.4775). Through this experiment, it is suggested that there is a loss of P15 protein during the development and progression of NPC. P15 protein expression can be used as a reference index for the biological behavior of NPC.