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采用淋巴细胞杂交瘤技术，制备了针对ｒＨｕＩＦＮα２ａ的单克隆抗体（ｍＡｂ）杂交瘤细胞系３Ｆ１，对ｍＡｂ３Ｆ１识别ｒＨｕＩＦＮα２ａ表位、中和活性等特点进行了研究，并与ＣｅｌＴｅｃｈ公司的ｍＡｂＮＫ２做了平行比较。发现ｍＡｂ３Ｆ１与ｍＡｂＮＫ２均具有中和ｒＨｕＩＦＮα２ａ活性的作用，但二者识别ｒＨｕＩＦＮα２ａ分子的表位不同，表明在ｒＨｕＩＦＮα２ａ分子上至少有２个中和性表位。进一步研究发现，ｍＡｂ３Ｆ１具有良好的用于亲和层析纯化ｒＨｕＩＦＮα２ａ的性能，每毫升湿胶可回收０．８ｍｇ１．０ｍｇｒＨｕＩＦＮα２ａ，纯度达９５％以上，生物学活性达２×１０１１ｕ／ｇ，且ｍＡｂ３Ｆ１有助于ｒＨｕＩＦＮα２ａ的复性。提示ｒＨｕＩＦＮα２ａ分子上的中和性表位，在与ｍＡｂ３Ｆ１的结合和解离过程中，对于ｒＨｕＩＦＮα２ａ的进一步复性起到促进作用
Monoclonal antibody (mAb) hybridoma cell line 3F1 against rHuIFNα2a was prepared by using lymphocyte hybridoma technology. The mAb 3F1 recognition rHuIFN α2a epitope, neutralizing activity and other characteristics were studied, and with the CelTech mAbNK2 Made a parallel comparison. Found mAb3F1 and mAbNK2 have the role of neutralizing rHuIFN α2a activity, but the two identify rHuIFN α2a epitopes of different molecules, indicating that in the rHuIFN α2a molecules at least two neutralizing epitopes. Further study found that mAb3F1 has good performance for affinity purification rHuIFN α2a per milligram wet glue can be recovered 0.8mg1.0mgrHuIFN α2a purity of 95% or more, biological activity of 2 × 1011u / g , And mAb3F1 help rHuIFN α2a refolding. Tip rHuIFN α2a neutralizing epitopes on the molecule, with mAb3F1 binding and dissociation process, for rHuIFN α2a further refolding play a catalytic role