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目的:通过比较不同加工方法制得的北沙参中粗多糖(Polysaccharide of Radix Glehnia,GLP)对阴虚小鼠的免疫调节作用,探讨北沙参去皮和不去皮的意义。方法:采用去皮和不去皮2种方法加工北沙参,分别提取去皮北沙参中的粗多糖(GLP1)和未去皮北沙参中的粗多糖(GLP2);制备阴虚小鼠模型,观察小鼠体质量变化,检测其脾脏抗体生成细胞(AFC)、迟发型超敏反应(DTH)和腹腔巨噬细胞(MΦ)吞噬百分率、吞噬指数等免疫指标。结果:GLP1和GLP2均可使阴虚小鼠体质量显著增加(P<0.05或P<0.01);亦能显著促进阴虚小鼠脾脏AFC的生成(P<0.05或P<0.01);增强DTH反应(P<0.01);对腹腔MΦ的吞噬百分率和吞噬指数无明显影响(P>0.05);且2种北沙参粗多糖(GLP1、GLP2)各相同剂量组之间的作用无显著性差异(P>0.05)。结论:2种北沙参粗多糖GLP1和GLP2在增强机体免疫功能方面无显著性差异,说明北沙参可以不去皮应用,为改进北沙参的传统加工方法提供了实验依据。
OBJECTIVE: To compare the immunomodulatory effects of Polysaccharide of Radix Glehnia (GLP) on mice with yin deficiency caused by different processing methods, and to explore the significance of skinless and non-skinlessness of Radix isatidis. Methods: Two kinds of methods, peeled and non-peeled, were used to process Beigia ginseng. The crude polysaccharides (GLP1) and the non-peeled ginseng polysaccharides (GLP2) Mouse model to observe the changes of the body weight of mice and detect the immune indexes such as phagocytic percentage and phagocytic index of AFC, DTH and MΦ. Results: Both GLP1 and GLP2 could significantly increase the body weight of mice with yin deficiency (P <0.05 or P <0.01), and also significantly promote the formation of AFC (P <0.05 or P <0.01) (P <0.01). There was no significant difference in the phagocytic percentage and phagocytic index of peritoneal MΦ (P> 0.05). There was no significant difference between the two groups in the same dose of GLP1 and GLP2 (P> 0.05). CONCLUSION: There is no significant difference between the two polysaccharides of Panax notoginseng GLP1 and GLP2 in enhancing immune function, which indicates that Panax notoginseng can be applied without peeling, which provides the experimental evidence for improving the traditional processing methods of Radix isatidis.