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目的:探讨二苯乙烯苷(2,3,5,4’-tetrahydroxystibene-2-O-β-D-glucoside,TSG)对H2O2诱导的PC12细胞损伤的抗凋亡作用。方法:本实验分为正常对照组、H2 O2处理组和H2 O2+TSG(0.1,1,10和50μmol.L-1)预处理组。采用MTT比色法和乳酸脱氢酶(LDH)测定法检测细胞活性,Hoechst染色观察细胞凋亡程度,Western Blotting检测细胞凋亡相关蛋白表达。结果:与正常对照组相比,细胞经H2O2诱导处理后,细胞存活率降低,LDH释放增加,Hoechst染色显示凋亡细胞增多,细胞核呈固缩突亮或碎块状致密浓染,Western Blotting显示促凋亡蛋白Bax表达升高,抑凋亡蛋白Bcl-2表达降低,Bcl-2与Bax的比值降低。不同浓度TSG预处理能改善H2O2所致的细胞存活率降低,减轻细胞凋亡程度,减少促凋亡蛋白Bax的表达以及增加抑凋亡蛋白Bcl-2的表达。结论:TSG可抑制H2O2诱导的PC12细胞损伤,其机制可能与抗凋亡有关。
Objective: To investigate the anti-apoptotic effects of 2,3,5,4’-tetrahydroxystibene-2-O-β-D-glucoside (TSG) on H2O2-induced PC12 cell injury. Methods: The experiment was divided into normal control group, H2O2 treatment group and H2O2 + TSG (0.1,1,10 and 50μmol.L-1) pretreatment group. Cell viability was measured by MTT colorimetric assay and lactate dehydrogenase (LDH) assay. Hoechst staining was used to observe the degree of apoptosis. Western blotting was used to detect the expression of apoptosis-related proteins. Results: Compared with the normal control group, the cell viability decreased and the release of LDH increased after H 2 O 2 induction. The Hoechst staining showed that the apoptotic cells increased, the nuclei were pyknotic or fragmented, and the results of Western Blotting The pro-apoptotic protein Bax increased, the anti-apoptotic protein Bcl-2 decreased, and the ratio of Bcl-2 to Bax decreased. Pretreatment with different concentrations of TSG could reduce the cell viability induced by H2O2, reduce the degree of apoptosis, decrease the expression of pro-apoptotic protein Bax and increase the expression of anti-apoptotic protein Bcl-2. Conclusion: TSG can inhibit H2O2-induced PC12 cell injury, and its mechanism may be related to anti-apoptosis.