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我们在改进病毒电镜制样方法时(注:请参见本刊本期中《乳胶溶液电镜法在检测植物病毒中的应用》一文)遇到这样的问题,即要将改进的乳胶溶液电镜法所制样品其铜网所捕获的病毒数与直沾法和免疫电镜法的相比较,以确定其灵敏度提高倍数。我们采用的是在使用相同规格铜网的前提下,将每种方法所制样品中各个网格所捕获病毒的平均数进行比较。各网格捕获病毒平均数按如下方法得来:依抽样原理,在适当而确定的
We are in the process of improving the virus electron microscopy sample preparation method (Note: see this issue of “latex solution electron microscopy in the detection of plant virus application” article) encountered this problem, that is, to be improved latex solution electron microscopy The number of viruses captured by the copper mesh of the samples was compared with that of the direct smears and the immunoelectron microscopy to determine the increase of sensitivity. We used to compare the average number of viruses captured by each grid in each method sample, using the same size copper mesh. The average number of viruses captured by each grid is obtained as follows: According to the sampling principle,