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临床工作中,一些根尖周炎病例久治不愈,常称为难治性根尖周炎。以往有研究表明,少数这种病例可分离出酵母菌。本实验目的是通过更加综合、完善的实验研究,进一步揭示酵母菌在难治性根尖周炎中的作用。 收集芬兰全国4年间共967例难治性根尖周炎病例,以插入根管并达根尖部的纸捻作为研究标本。每个标本经匀浆处理、稀释后,分别作有氧、兼性厌氧和厌氧培养各10d,并经过严格的酵母菌和细菌筛选、鉴定实验。 研究结果如下:①47个标本(7％)分离出48个真菌菌株,均为内孢霉菌属的酵母菌,其中6个(13％)为真菌纯培养,其余41个标本见与细菌共存;②48个菌株中可鉴别出菌种20个,除1例为白地霉菌外均属念
In clinical work, some cases of periapical periodontitis, often referred to as refractory apical periodontitis. Previous studies have shown that a small number of such cases can be isolated yeast. The purpose of this experiment is to further reveal the role of yeast in refractory apical periodontitis through a more comprehensive and comprehensive experimental study. A total of 967 cases of refractory periapical periodontitis were collected during the four-year period in Finland. The paper twines inserted into the root canal and reached the root tip were used as research samples. Each sample was homogenized, diluted, were aerobic, facultative anaerobic and anaerobic culture of the 10d, and after strict screening of yeast and bacteria, identification experiments. The results were as follows: ① Forty-eight strains of fungi were isolated from 47 specimens (7%), all of them were Saccharomyces cerevisiae, of which 6 (13%) were pure fungi and the other 41 were found to coexist with bacteria; A strain of bacteria can be identified in 20, except for 1 case of white mold outside are read