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本文以 CD光谱及巯基测定两种方法监测了肌酸激酶的变性过程.快速可反应巯基的测定表明,巯基暴露速度与从前报道过的荧光光谱及紫外差光谱的变化速度大体相当.但是,在低浓度胍溶液中,隐蔽的芳香氨基酸尚未暴露时,酶分子的椭圆度值已有了明显可察的变化.变性速度的比较表明,220nm平均残基椭圆度值的变化速度明显地慢于芳香氨基酸、巯基的暴露速度.这可能意味着,肌酸激酶分子内某些肽段具有松散的二级结构,又靠近分子表面,易受到低浓度胍的变性作用.而整个酶分子的二级有序结构的破坏,还是慢于卷曲肽链的伸展速度,也即三级结构的破坏速度。
In this paper, CD spectroscopy and thiol determination of two methods to monitor the degeneration of creatine kinase rapid reaction can be measured by mercapto, mercapto exposure rate and the previously reported fluorescence spectra and UV spectra of the rate of change roughly the same, but in Low concentrations of guanidine solution, the concealed aromatic amino acids have not been exposed, the enzyme molecule ovality value has been significantly observed changes in the comparison of the denaturation rate, 220nm average residue ellipticity value of the rate of change is significantly slower than the aromatic Amino acids, sulfhydryl exposure rate.This may mean that some peptides within the creatine kinase molecule have a loose secondary structure, close to the surface of the molecule, susceptible to low concentrations of guanidine denaturation and the entire enzyme molecules of the secondary Orbital structure damage, or slower than curly peptide chain stretching speed, that is, the destruction rate of tertiary structure.