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目的研究雌激素替代治疗(estrogenreplacementtherapy,ERT)对去卵巢骨质疏松(ovariectomizedosteoporosis,OVX-OP)大鼠腺垂体滤泡星形(folliculo-stellate,FS)细胞中神经组织蛋白S-100、间隙连接蛋白Cx43表达的影响。方法采用10月龄、未孕产SD雌性大鼠60只,随机分为卵巢切除组(OVX)、假性手术对照组(Sham)和卵巢切除+雌激素替代治疗组(OVX+ERT),每组20只。于术后6周末应用双能X线骨吸收测量法(DEXA)检测Sham组和OVX组大鼠L4～6骨密度(bonemin-eraldensity,BMD)。OVX+ERT组大鼠于OVX术后第7～12周按照每周1mg/kg体重的剂量给予尼尔雌醇,于术后12周末检测大鼠L4～6BMD。制备各组大鼠腺垂体标本切片,应用间接免疫荧光法结合激光扫描共聚焦显微镜(LSCM)定位和定量分析腺垂体FS细胞中S-100、Cx43的表达。结果术后6周末Sham组L4～6BMD(0.211±0.013)g/cm2及术后12周末的OVX+ERT组L4～6BMD(0.204±0.010)g/cm2均明显高于术后6周末OVX组L4～6BMD(0.193±0.006)g/cm2(P<0.01)。S-100蛋白定位于FS细胞的胞浆中,Sham组、OVX组和OVX+ERT组中S-100蛋白表达的阳性率分别为80%、85%和85%,差异无显著性意义(P>0.05)。Cx43蛋白免疫荧光阳性信号定位于相邻的FS细胞以及相邻的FS细胞与内分泌细胞的胞浆中和(或)胞膜上。Sham
Objective To investigate the effect of estrogen replacement therapy (ERT) on the expression of neural tissue protein S-100 in the folliculo-stellate (FS) cells of ovariectomized osteoporosis (OVX-OP) Effect of protein Cx43 expression. Methods Sixty female SD rats of 10 months old and without pregnancy were randomly divided into two groups: ovariectomized group (OVX), sham operated group (Sham) and ovariectomized + estrogen replacement group (OVX + ERT) Group of 20. At 6 weeks after operation, bone mineral density (BMD) of L4 ~ 6 in sham group and OVX group was detected by dual energy X-ray absorptiometry (DEXA). The rats in OVX + ERT group were given nilestriol at the dose of 1 mg / kg body weight every week 7 to 12 weeks after OVX. Rats L4 ~ 6BMD were detected at the end of the 12th week. The adenohypophyseal sections were prepared and the expression of S-100 and Cx43 in pituitary FS cells was detected by indirect immunofluorescence combined with laser confocal laser scanning microscopy (LSCM). Results L4 ~ 6BMD (0.211 ± 0.013) g / cm2 in Sham group and 0.204 ± 0.010 g / cm2 in OVX + ERT group at 6 weeks after operation were significantly higher than those in OVX group at 6 weeks after operation ~ 6BMD (0.193 ± 0.006) g / cm2 (P <0.01). The positive rates of S-100 protein in Sham group, OVX group and OVX + ERT group were 80%, 85% and 85%, respectively, and the difference was not significant (P > 0.05). Immunofluorescence positive signals of Cx43 protein are located in the cytoplasm and / or membrane of adjacent FS cells and adjacent FS cells and endocrine cells. Sham