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采用单个体养殖实验和生化分析技术,研究了纯化饲料(A组)、活饵料(章鱼和缢蛏,B组)和混合投喂(纯化饲料和活饵料有规律地交替使用,C组)对三疣梭子蟹幼蟹(初体重0.5 g左右)成活、生长性能、肝胰腺指数(HSI)和生化组成的影响。结果表明:(1)3组幼蟹的成活率并无显著差异,均在70%～80%之间;(2)A组和B组幼蟹的增重率、蜕壳周期、特定增长率和饲料系数无显著差异,C组幼蟹的第二次蜕壳周期和饲料系数显著低于其它两组,其特定增长率显著高于其它两组;(3)3种投喂方式的饵料对幼蟹的肝胰腺指数及其蛋白和脂肪含量影响显著,A组幼蟹肝胰腺指数及其脂肪含量显著高于其它两组,但蛋白含量显著低于其它两组;(4)就脂肪酸组成而言,A组肝胰腺和躯体中的饱和脂肪酸和单不饱和脂肪酸均高于其它两组,多不饱和脂肪酸和高度不饱和脂肪酸显著低于其它两组。因此,研制的纯化饲料可以使三疣梭子蟹幼蟹获得较好的生长性能,为进一步开展梭子蟹幼蟹营养需求研究奠定了良好的基础。
The effects of purified feed (group A), live bait (octopus and emu, group B) and mixed fed (regular alternation of purified feed and live bait, group C) were studied using single-body culture experiments and biochemical analysis techniques. Survival, growth performance, hepatopancreas index (HSI) and biochemical composition of crab crab crab (initial body weight about 0.5 g). The results showed that: (1) There was no significant difference in the survival rates between the three groups of crabs, both between 70% and 80%; (2) The weight gain rate, molt cycle and specific growth rate And the feed coefficient had no significant difference. The second demulsification period and feed coefficient of crab group C were significantly lower than the other two groups, and the specific growth rate was significantly higher than the other two groups. (3) The index of hepatopancreas and its protein and fat content in juvenile crabs were significantly affected. The index of hepatopancreas and its fat content in juvenile group A were significantly higher than those in the other two groups, but the protein content was significantly lower than that in the other two groups. (4) Say, a group of liver and pancreas and body of saturated fatty acids and monounsaturated fatty acids were higher than the other two groups, polyunsaturated fatty acids and highly unsaturated fatty acids were significantly lower than the other two groups. Therefore, the developed feed can make Portunus tritubus larvae obtain better growth performance, which lays a good foundation for the further research on the nutritional requirements of the larvae.