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本文研究了2-(2-噻唑偶氮)-对-甲酚(TAC)与镍(Ⅱ)、钴(Ⅱ)螯合物的柱前衍生条件和液相色谱的分离条件。于KYWG-C_(18)柱上,用含有0.03mol/L KH_2PO_4-Na_2HPO_4(pH7.77)和2.5×10~(-4)mol/L TAC的甲醇-四氢呋喃-水(40∶10∶50 V/V)混合液为流动相,流速为1.0mL/min。于580nm处检测。该法用于四种标准合金中镍和钴的测定,回收率在98%～101.5%之间。Ni(Ⅱ)和Co(Ⅱ)的检出限(信噪比为2∶1)分别为0.2ng和1.5ng。建立了同时测定镍,钴的高灵敏、高选择性,快速的HPLC-光度法。
In this paper, pre-column derivatization conditions and liquid chromatography separation conditions of 2- (2-thiazolylazo) -p-cresol (TAC) and nickel (Ⅱ) and cobalt (Ⅱ) chelates were studied. On a KYWG-C 18 column, the column was eluted with methanol-tetrahydrofuran-water (40:10:50 V) containing 0.03 mol / L KH 2 PO 4 -H 2 HPO 4 (pH 7.77) and 2.5 × 10 -4 mol / L TAC / V) mixture as mobile phase at a flow rate of 1.0 mL / min. Detection at 580nm. This method is used for the determination of nickel and cobalt in four standard alloys with recoveries between 98% and 101.5%. The detection limits (signal to noise ratio of 2: 1) of Ni (Ⅱ) and Co (Ⅱ) were 0.2ng and 1.5ng, respectively. The simultaneous determination of nickel, cobalt, high sensitivity, high selectivity, fast HPLC-spectrophotometry.