论文部分内容阅读
目的检测慢性阻塞性肺疾病(COPD)患者血清中一氧化氮(NO)的变化,并探讨NO与肿瘤坏死因子-α(TNF-α)、超敏C-反应蛋白(hs-CRP)的相关性。方法采集COPD急性加重期、稳定期患者和健康者外周血血清,采用硝酸还原酶法检测血清中NO、速率散射比浊法检测血清hs-CRP和放射免疫法定量测定血清TNF-α浓度。结果 COPD急性加重期组NO水平明显低于稳定期组(P<0.01),而TNF-α和hs-CRP显著高于稳定期组(P<0.01);COPD稳定期组NO水平明显低于对照组(P<0.05),TNF-α水平明显高于对照组(P<0.05),而hs-CRP水平在稳定期组和对照组之间比较差异无统计学意义(P>0.05);COPD急性加重期NO与TNF-α、hs-CRP存在明显负相关性(r=-0.365,P=0.047,r=-0.450,P=0.013)。COPD稳定期患者血清NO与TNF-α、hs-CRP之间无明显相关性(r=-0.148,P=0.434;r=-0.09,P=0.636)。结论 NO、TNF-α、hs-CRP均参与了COPD气道炎性反应,并与COPD炎性损伤程度有关。此外,NO水平越低,炎性反应越剧烈,提示NO在炎性级联反应中起一定作用。
Objective To investigate the changes of serum nitric oxide (NO) in patients with chronic obstructive pulmonary disease (COPD) and to explore the correlation between NO and tumor necrosis factor-α (TNF-α) and hs-CRP Sex. Methods Serum levels of peripheral blood serum of COPD patients and healthy controls were measured. Nitric acid reductase was used to detect serum NO. Serum hs-CRP was detected by nephelometry. Radioimmunoassay was used to determine serum TNF-α levels. Results The NO level in COPD acute exacerbation group was significantly lower than that in stable phase group (P <0.01), while TNF-α and hs-CRP were significantly higher than those in stable phase group (P <0.01) (P <0.05). The level of TNF-α was significantly higher than that of the control group (P <0.05), while the level of hs-CRP was not significantly different between the stable group and the control group (P> 0.05) There was a significant negative correlation between NO and TNF-α and hs-CRP in exacerbation stage (r = -0.365, P = 0.047, r = -0.450, P = 0.013). There was no significant correlation between serum NO and TNF-α and hs-CRP in stable COPD patients (r = -0.148, P = 0.434; r = -0.09, P = 0.636). Conclusion NO, TNF-α and hs-CRP are all involved in the airway inflammatory response in COPD and are related to the degree of inflammatory injury in COPD. In addition, the lower the NO level, the more intense the inflammatory response, suggesting that NO plays a role in the inflammatory cascade.