模拟8000m低氧对大鼠脑内环核苷酸含量的影响

• 作 者: 汤燕平 白若华 肖惠宁 樊凌冰
• 机 构: 青海医学院生理教研室，青海医学院附属医院同位素室
• 出 处: 高原医学杂志
• 发表日期: 1995年02期
• 关键词: 环核苷酸 低氧 鼠脑 缺氧反应 减压舱 高山医学 青海医学院 磷酸腺苷 内环 皮层细胞

利用减压舱模拟８０００ｍ低氧和放射免疫的方法，观察了大鼠脑内环—磷酸腺苷（ｃＡＭＰ）和环—磷酸鸟鲁（ｃＧＭＰ）含量的变化。以西宁地区的海拔２２６０ｍ作为对照，并进行比较。结果发现，海拔８０００ｍ低氧时，大鼠脑内ｃＡＭＰ含量明显升高。同海拔高度比较，小脑ｃＡＭＰ的含量明显高于大脑皮层内的含量（Ｐ＜０．０１）。尽管小脑ｃＧＭＰ的含量在８０００ｍ组明显升高，但在同海拔高度小脑与大脑皮层ｃＧＭＰ的含量间无显著性差异。本研究结果提示脑内环核苷酸的含量是脑缺氧反应的敏感指标，而且以ｃＡＭＰ的反应更灵敏。

The changes of cAMP and cGMP contents in the brain of rats were observed by hypoxia chamber simulation of 8000 m hypoxia and radioimmunoassay. The Xining area elevation 2260m as a control, and compared. The results showed that, at altitude 8000m hypoxia, the rat brain cAMP content was significantly increased. Compared with altitude, the content of cAMP in cerebellum was significantly higher than that in cerebral cortex (P <0.01). Although the content of cGMP in cerebellum was significantly increased in 8000m group, there was no significant difference in the content of cGMP between cerebellar and cerebral cortex at the same altitude. Our results suggest that intracytoplasmic nucleotide content is a sensitive indicator of cerebral hypoxia, and the response to cAMP is more sensitive.