论文部分内容阅读
目的:观察含血清和无血清培养的星形胶质细胞(Ast)经不同时程糖氧剥夺(OGD)孵育后其释放细胞因子功能的变化。方法:体外培养Ast,分为对照组和OGD组,各组再分为含血清和无血清培养不同时程亚组(0、3、6、9、12、16和24h组)。用MTT法测定不同培养条件和时程的Ast相对活性,ELISA法检测Ast释放到培养液中的促炎因子(TNF-α、IL-1β、IL-6)和粒细胞集落刺激因子(G-CSF)的水平。结果:①OGD处理9h:含血清培养的Ast仍然保持高效的增殖能力,而无血清培养的Ast增殖能力明显受抑。②OGD处理24h:含血清培养的Ast存活率高于50%,无血清培养的Ast存活率约30%。③OGD处理24h:含血清培养的Ast最早释放增加的细胞因子为G-CSF,次之为IL-6;TNF-α和IL-1β无释放增加的趋势;无血清培养的Ast基本无G-CSF释放增加,其他3种因子均于OGD处理3h开始释放增加,仅持续时间长短不同。结论:OGD处理9h内,含血清培养的Ast仍然可以保持高效的增殖,而无血清培养的Ast增殖明显受抑;Ast释放G-CSF和促炎因子的时段与它们所处的培养环境密切相关。
OBJECTIVE: To observe the changes of cytokines released by astrocytes (Ast) cultured in serum-containing and serum-free medium after OGD incubation for different time courses. Methods: Ast was cultured in vitro and divided into control group and OGD group. Each group was subdivided into subgroups with different durations (0, 3, 6, 9, 12, 16 and 24 h). The relative activity of Ast in different culture conditions and time course was determined by MTT assay. The levels of proinflammatory cytokines (TNF-α, IL-1β, IL-6) and granulocyte colony stimulating factor (G- CSF) levels. Results: ①OGD treatment 9h: serum-containing Ast still maintain high proliferative capacity, while serum-free culture of Ast proliferation was significantly inhibited. ②OGD treatment 24h: serum-containing Ast survival rate above 50%, Ast serum serum-free survival of about 30%. (3) OGD treatment 24h: Serum-containing Ast was the first to release G-CSF, followed by IL-6; no release of TNF-α and IL-1β. Serum- Release increased, the other three kinds of factors were released in the OGD treatment began to increase 3h, only the duration of different lengths. CONCLUSION: Astragalus can still maintain high proliferation within 8 hours after OGD treatment, while asthma in serum-free culture is significantly inhibited. The time course of releasing G-CSF and proinflammatory factors from Astragalus mongholicus is closely related to their culture environment .