论文部分内容阅读
目的 探讨补体成分在羊水栓塞中的变化及病理生理作用。方法 2 0 0 4年 1～ 5月中国医科大学附属二院精选雌性Wistar大鼠 30只于妊娠 2 0d制作羊水栓塞模型。根据注入液体性质不同随机分为对照组 (10只 )、羊水组 (10只 )、胎粪液组 (10只 )。对照组除注入生理盐水外 ,皆与另 2组条件相同。实验后取大鼠左肺行HE染色光镜下观察 ,免疫比浊法测各组注入前后的补体C3 、C4水平。结果 实验组肺组织可见大量炎性细胞浸润 ,包括白细胞 (主要为中性粒细胞 )、巨噬细胞及少量淋巴细胞。对照组注入前后补体C3 、C4水平比较差异无显著性意义 (P >0 0 5 ) ;羊水组和胎粪液组注入前后补体C3 、C4水平明显降低 ,差异有显著性意义 (P <0 0 1)。结论 补体系统在羊水栓塞中被大量激活、消耗 ,其活化裂解产生的片段引起中性粒细胞、巨噬细胞浸润及一系列病理变化 ,由此引起肺损伤及临床症状。
Objective To investigate the changes of complement components in amniotic fluid embolism and its pathophysiological effects. METHODS: From January to May 2004, 30 female Wistar rats from the Second Affiliated Hospital of China Medical University were enrolled in the study. According to the different injection of liquid properties were randomly divided into control group (10), amniotic fluid group (10), meconium solution group (10). The control group except the injection of saline, the same conditions with the other two groups. After the experiment, the left lungs of rats were observed under light microscope and the levels of complement C3 and C4 before and after injection were measured by immunoturbidimetry. Results The experimental group showed a large number of inflammatory cell infiltration of lung tissue, including leukocytes (mainly neutrophils), macrophages and a small amount of lymphocytes. The levels of complement C3 and C4 in the control group were not significantly different (P> 0.05). The level of complement C3 and C4 in the amniotic fluid and meconium group was significantly lower than that before injection (P <0 0) 1). Conclusion The complement system is largely activated and depleted in amniotic fluid embolism. The fragments produced by its activation and cleavage cause neutrophil and macrophage infiltration and a series of pathological changes, which lead to lung injury and clinical symptoms.