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目的探讨高糖(HG)及氟伐他汀对人肾小管上皮细胞2(HK-2)klotho蛋白表达的影响及机制。方法正常糖(NG,5.5mmol/L),HG(25.0mmol/L)培养HK-2,不同浓度氟伐他汀(0.1、1.0、10μmol/L)伴/不伴甲羟戊酸(MVA)、焦磷酸牛龙牛儿基牛龙牛儿酯(GGPP)、焦磷酸法尼酯(FPP),干预不同时间(0、12、24、48h),Western blot检测klotho、RhoA蛋白表达。结果与NG培养比较,HG刺激HK-2表达klotho蛋白量明显减少(P<0.05);氟伐他汀干预后部分恢复(与HG刺激比较,P<0.05);此作用可被MVA、GGPP逆转,FPP不能逆转。HK-2RhoA蛋白量在HG刺激下增加(与NG培养比较,P<0.05),氟伐他汀干预后表达减少(P<0.05)。结论 HG抑制HK-2klotho蛋白表达;氟伐他汀上调HK-2表达klotho蛋白,机理可能与其抑制RhoA/Rho激酶信号通路有关。
Objective To investigate the effect of high glucose (HG) and fluvastatin on klotho protein expression in human renal tubular epithelial cells 2 (HK-2) and its mechanism. Methods HK-2 was cultured in normal glucose (NG, 5.5mmol / L) and HG (25.0mmol / L) with different concentrations of fluvastatin (0.1,1.0,10μmol / L) with / without mevalonate (MVA) GGPP and FPP were taken at different times (0, 12, 24 and 48 h) respectively. Western blot was used to detect the expression of klotho and RhoA. Results Compared with NG culture, the amount of klotho protein in HG-stimulated HK-2 expression was significantly decreased (P <0.05); fluvastatin partially recovered after HGF stimulation (P <0.05 compared with HG stimulation); this effect was reversed by MVA and GGPP, FPP can not be reversed. The amount of HK-2RhoA protein was increased under HG stimulation (compared with NG culture, P <0.05), and decreased after fluvastatin treatment (P <0.05). Conclusions HG can inhibit the expression of HK-2klotho protein. Fluvastatin can increase the expression of klotho protein by HK-2, which may be related to the inhibition of RhoA / Rho kinase signaling pathway.