Soluble Expression in Escherichia Coli and Purification of Human Carboxylesterase 1

• 作 者: WANG Lei TONG Jin-ying CAO Pen
• 机 构: MedicalCollegeofHunanNormalUniversity,ChangshaYingrunBiotechnologiesInc.
• 出 处: 中国生物医学工程学报：英文版
• 发表日期: 2014年1期
• 关键词: 可溶性表达 大肠杆菌 羧酸酯酶 纯化 WESTERN-BLOT WESTERN印迹 ELISA方法 单克隆抗体 hCE 1 Escherichia coli
• 基金项目: The National Natural Science Foundation of China，grant number：31170092，grant sponsor：Hunan Provincial Natural Science Founda-tion of China，grant number：13JJ2021

Objective: To achieve an optimized method for soluble expression of human carboxylesterase 1(hCE-1) in escherichia coil and purification by Ni2+-NTA agarose affinity chromatography, to get improved protein yield and purity for further development of hepat