REPRODUCTIVE MECHANISM OF RUDITAPES PHILIPPINARUM ISOLATION OF SHEDDING HORMONE AND DETERMINATION OF

• 作 者: 方永强 张玉生 许瑞安 张其森
• 机 构: Third Institute of Oceanography, National Bureau of Oceanography, Xiamenrrrrrrrrn,Third Ins
• 出 处: Acta Oceanologica Sinica
• 发表日期: 1985年01期
• 关键词: ammonia sorts capable precipitate dissolved preliminarily parent granules analyz

An active substance, shedding hormone capable of inducing parent clams to spawn and sper-miate, has preliminarily been isolated from the ovaries of Ruditapes philippinarum by means of ammonia sulfate precipitate of 25% concentration and Sephadex G-25 column chromatography in present study. The biological activity of the shedding hormone is above 95%. It is composed of sorts of amino acids by determination with an amino acid automatic analyzer. The experimental results indicate that the active substance is a polypeptide.

An active substance, shedding hormone capable of inducing parent clams to spawn and sper-miate, has preliminarily been isolated from the ovaries of Ruditapes philippinarum by means of ammonia sulfate precipitate of 25% concentration and Sephadex G-25 column chromatography in present study. The biological activity of the shedding hormone is above 95%. It is composed of sorts of amino acids by determination with an amino acid automatic analyzer. The experimental results that that the active substance is a polypeptide.