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目的 探讨原发性食管鳞状细胞癌中血管内皮生长因子 (VEGF)的表达情况及其与肿瘤血管生成和预后的关系。方法 采用免疫组织化学染色方法 ,分别以抗VEGF多克隆抗体和抗F VIII单克隆抗体检测 82例手术切除标本中VEGF的表达水平和肿瘤微血管密度 (MVD)值 ,分析二者之间及与患者预后的相关性。结果 全组病例中 ,肿瘤细胞VEGF阳性率为 6 3 4% (5 2 / 82 )VEGF阴性和VEGF阳性的 3年和 5年生存率分别为 5 3 4%和 46 6 % ;19 2 %和 11 5 %。MVD中位值为37个 /mm2 (9～ 15 0个 /mm2 )。VEGF的表达水平与MVD值密切相关 (P =0 0 0 1)。MVD与患者的预后显著相关 (P =0 0 17)。随着VEGF表达水平增高 ,患者预后明显恶化 (P =0 0 0 0 )。结论 VEGF在食管鳞状细胞癌的肿瘤血管生成过程中发挥重要的调控作用。多因素预后分析表明VEGF的表达可作为食管鳞状细胞癌患者独立的预后判断指标。
Objective To investigate the expression of vascular endothelial growth factor (VEGF) in primary esophageal squamous cell carcinoma and its relationship with tumor angiogenesis and prognosis. Methods Immunohistochemical staining was used to detect VEGF expression and tumor microvessel density (MVD) in 82 surgically resected specimens with anti-VEGF polyclonal antibody and anti-F VIII monoclonal antibody respectively. Correlation of prognosis. Results The 3-year and 5-year survival rates of VEGF-positive and VEGF-positive were 63.4% (52/82) in all the cases. The 3-year and 5-year survival rates were 53.4% and 46.6% 11 5%. The median MVD was 37 per mm2 (9-1500 per mm2). The expression of VEGF was closely related to MVD (P = 0 0 01). MVD was significantly associated with the patient’s prognosis (P = 0 0 17). With the increase of VEGF expression, the prognosis of patients was significantly worse (P = 0.0000). Conclusion VEGF plays an important regulatory role in the tumor angiogenesis of esophageal squamous cell carcinoma. Multivariate prognostic analysis showed that the expression of VEGF can be used as an independent prognostic indicator in patients with esophageal squamous cell carcinoma.