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大鼠全脑反复缺血后产生不可逆性学习记忆障碍的研究，采用４血管阻断的方法，脑反复缺血再灌流，然后长期饲养。作水迷宫试验和跳台试验，结果显示缺血后出现了显著的、不可逆的学习记忆障碍。同时用放射免疫方法测定了额叶、颞叶、纹状体、丘脑、海马、Ｍｅｙｎｅｒｔ氏核中胆碱递质和ＡＶＰ，发现ＡＣｈ、ＣｈＡＴ、ＡＶＰ含量显著下降。下降的时间、幅度一致，变化呈正相关。ＡＣｈ、ＣｈＡＴ、ＡＶＰ是与学习、记忆密切相关的神经递质和神经肽。因而推断脑缺血后出现不可逆性学习记忆障碍与多脑区内ＡＣｈ、ＣｈＡＴ、ＡＶＰ含量显著下降有关。这对探讨临床上血管性痴呆发病机理进而开发有效的防治药物有重要意义。
After repeated brain ischemia in rats, irreversible learning and memory impairment occurred. The method of 4-vessel occlusion was used. The brain was repeatedly subjected to ischemia-reperfusion and then reared for long-term. Water maze test and platform test, the results showed that after ischemia there was a significant, irreversible learning and memory disorders. The contents of ACh, ChAT and AVP in frontal lobe, temporal lobe, striatum, thalamus, hippocampus and Meynert’s nucleus were measured by radioimmunoassay. Declining time, the same magnitude, the change was positively correlated. ACh, ChAT and AVP are neurotransmitters and neuropeptides that are closely related to learning and memory. Therefore, it is concluded that the irreversible learning and memory impairment after cerebral ischemia is related to the significant decrease of ACh, ChAT and AVP contents in multiple brain regions. This is of great significance in exploring the pathogenesis of clinical vascular dementia and developing effective anti-inflammatory drugs.