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目的观察原发性高血压患者血浆孤啡肽水平的改变及孤啡肽对血管内皮舒张因子（ＥＤＲＦ／ＮＯ）的影响，初步探讨孤啡肽舒张血管，降低血压的可能机制。方法应用放射免疫测定法测定原发性高血压患者（３２例）血浆孤啡肽水平并以正常成年人（１９例）作对照。大鼠主动脉条组织孵育液中亚硝酸盐的含量可反映一氧化氮（ＮＯ）的产生量，采用微盘测定法测定，用张新波等建立的ＮＯ合酶测定改良法检查ＮＯＳ活性。结果ＥＨ患者血浆孤啡肽水平明显高于对照组（１５．４７±２．１４ｎｇ／Ｌｖｓ８．８３±５．６８ｎｇ／Ｌ，Ｐ＜０．０５），且随高血压程度分期的加重，孤啡肽水平增加越明显。孤啡肽能明显刺激大鼠主动脉条组织ＮＯ产生量和提高ＮＯＳ活性，随剂量的增加，此作用更加明显（Ｐ＜０．０５）。结论推测孤啡肽可能为一种存在于外周血液中与血压调节有关的新型血管活性多肽，其与心血管疾病的关系有待进一步深入研究。
Objective To observe the changes of plasma orphanin level in patients with essential hypertension and the effect of orphanin on the endothelial cell relaxing factor (EDRF / NO), and to explore the possible mechanism of orphanin to relax blood vessels and lower blood pressure. Methods Plasma levels of orphanin were measured by radioimmunoassay in 32 patients with essential hypertension (n = 32) and normal controls (n = 19). The content of nitrite in the aortic strips of rat atrium could reflect the production of nitric oxide (NO). The activity of NOS was detected by microtiter plate assay and NO synthase assay developed by Zhang Xinbo et al. Results The level of plasma orphanin in EH patients was significantly higher than that in the control group (15.47 ± 2.14ng / L vs 8.83 ± 5.68ng / L, P <0.05). With the increase of stage of hypertension, Increased peptide levels more obvious. Orphanin significantly stimulated the production of NO in rat aortic strips and increased NOS activity. With the increase of dose, the effect was more obvious (P <0.05). Conclusions It is hypothesized that orphanin may be a novel vasoactive polypeptide that is involved in the regulation of blood pressure in the peripheral blood and its relationship with cardiovascular diseases needs to be further studied.