论文部分内容阅读
目的:比较子痫前期(PE)患者与正常孕妇血清和胎盘F as/F asL的表达,探讨F as/F asL系统的表达与子痫前期的关系。方法:选择40例子痫前期患者和39例正常孕妇作为研究对象,采用EL ISA方法测定血清sF as和sF asL水平;采用W estern B lot检测胎盘F as和F asL的蛋白含量。结果:子痫前期组血清sF as水平(2.11±0.95)m g/L高于正常对照组(1.57±0.60)m g/L,差异有显著性(P<0.05);子痫前期组血清sF asL水平(4.43±1.90)μg/L也显著高于正常对照组(3.48±1.53)μg/L,P<0.01;两组胎盘中均测得F as及F asL蛋白表达,但二者差异均无显著性(P>0.05)。结论:F as和F asL的改变与PE相关,血清游离F as和F asL增高对子痫前期的发生和发展可能起着重要作用,F as/F asL的异常表达导致妊娠免疫耐受不当可能是PE发病的重要机制之一。
Objective: To compare the expression of F as / F asL in serum and placenta of preeclampsia (PE) patients with normal pregnant women and to explore the relationship between F as / F asL system expression and preeclampsia. Methods: Forty pregnant women with preeclampsia and 39 normal pregnant women were enrolled in this study. Serum levels of sF as and sF asL were measured by ELISA. The protein levels of F as and F asL were detected by Western blot. Results: Serum sF as level in preeclampsia group (2.11 ± 0.95) mg / L was significantly higher than that in normal control group (1.57 ± 0.60) mg / L (P <0.05) (4.43 ± 1.90) μg / L was also significantly higher than that of the normal control group (3.48 ± 1.53) μg / L, P <0.01; F as and F asL protein expression were measured in both groups, but the difference was insignificant (P> 0.05). CONCLUSIONS: Changes in F as and F asL are associated with PE. Elevated serum free F as and F asL may play important roles in the development and progression of preeclampsia. Abnormal expression of F as / F as L may result in improper immune tolerance in pregnancy PE is one of the important pathogenesis.