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目的探讨卵巢癌组织中环氧合酶2(COX-2)和PTEN蛋白表达及其临床意义。方法免疫组化染色检测卵巢癌(A组,96例)和正常卵巢组织(B组,40例)COX-2和PTEN蛋白表达。结果 A组COX-2蛋白表达水平(IOD值)高于B组(94.6±33.3vs.41.5±14.5)(P<0.01),而PTEN表达水平低于B组(38.4±12.6vs.91.2±31.3)(P<0.01)。与高分化、无淋巴结转移和临床Ⅰ、Ⅱ期的卵巢癌比较,中、低分化、有淋巴结转移和临床Ⅲ、Ⅳ期卵巢癌中COX-2表达升高,PTEN表达降低(P<0.05)。卵巢癌组织中COX-2和PTEN的表达呈显著负相关(r=-0.506,P<0.05)。COX-2阴性患者的3年累积生存率为38.6%,高于COX-2阳性患者的22.6%(P<0.05);PTEN阴性患者的3年累积生存率为21.5%,低于PTEN阳性患者的56.4%(P<0.05)。结论卵巢癌组织中COX-2和PTEN蛋白的异常表达参与了卵巢癌的发生、发展过程,有可能作为卵巢癌分级、预后评估的新指标。
Objective To investigate the expression of cyclooxygenase 2 (COX-2) and PTEN in ovarian cancer and its clinical significance. Methods The expressions of COX-2 and PTEN protein in ovarian cancer (group A, 96 cases) and normal ovarian tissue (group B, 40 cases) were detected by immunohistochemistry. Results The expression of COX-2 in group A was higher than that in group B (94.6 ± 33.3 vs.41.5 ± 14.5) (P <0.01), while the expression of PTEN was lower than that in group B (38.4 ± 12.6 vs.91.2 ± 31.3 ) (P <0.01). Compared with well-differentiated, non-lymph node metastasis and clinical stages Ⅰ and Ⅱ ovarian cancer, the expression of COX-2 and the expression of PTEN decreased in moderate and poorly differentiated group with lymph node metastasis and clinical stage Ⅲ and Ⅳ ovarian cancer (P <0.05) . There was a significant negative correlation between the expression of COX-2 and PTEN in ovarian cancer (r = -0.506, P <0.05). The 3-year cumulative survival rate of COX-2 negative patients was 38.6%, which was higher than that of COX-2 positive patients (22.6%, P <0.05). The 3-year cumulative survival rate of PTEN negative patients was 21.5%, lower than that of PTEN positive patients 56.4% (P <0.05). Conclusion The abnormal expression of COX-2 and PTEN protein in ovarian cancer is involved in the occurrence and development of ovarian cancer and may be used as a new index to evaluate the grade and prognosis of ovarian cancer.