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我們利用电生理学方法,研究了乙醯胆碱对短声引起的耳蝸电位的影响,初步分析了耳蝸传出突触的传递机制。耳蝸电位采用圆窗引导。将Ach溶液(0.5×10~(-4)M)做耳蝸基轉鼓阶局部注射,視Ach局部处理前后CM和Ap(N_1)振幅的变化,結果表明: 1、Ach溶液(0.5×10~(-4)M)耳蝸基轉鼓阶注射0.3—1μl后,使Ap(N_1)振幅明显降低,Ach注射前Ap(N_1)振幅的均值为0.23±0.05mv;Ach注射后Ap(N_1)振幅的均值为0.06±0.03mv,Ap(N_1)振幅平均下降83%。实驗数据經統計学t測驗,p<0.001。此結果說明Ach对Ap(N_1)有明显地抑制效应。与Ap(N_1)振幅降低的同时其潛伏期平均延长3.97 msec,此結果与前人报导电刺激耳蝸传出纖維使Ap(N_1)振幅降低,潛伏期延长的抑制效果一致。2、Ach溶液(0.5×10~(-4)M)耳蝸基轉鼓阶注射0.3—1μl后,使CM振幅明显减小。Ach注射前CM振幅的均值为0.06±0.01mv;注射后CM振幅的均值为0.03±0.01mv,CM振幅平均降低80%。p<0.001。結果表明Ach对CM振幅有明显的抑制效应。与CM振幅降低的同时,其潛伏期平均延长0.49 msec。3、在我們的工作中观察到Ach对耳蝸电位的抑制效应,表現两种作用方式,一种是驟然抑制,抑制速度极快,类似Ach的N一样作用;另一类則表抑制緩慢、持久,类似Ach的M一样作用。因此,我們推测,似乎耳蝸corti氏器官的毛細胞,也可能存在Ach的两种受体。4、根据前人的工作以及我們的实驗研究,似乎可以設想,耳蝸传出尖触抑制效应可能是一种胆碱能传递机制。
We use electrophysiological methods to study the acetylcholine on the short-tone caused by the impact of the cochlear potential, initially analyzed the transmission mechanism of cochlear outgoing synapses. Cochlear potential using circular window guide. Ach solution (0.5 × 10 ~ (-4) M) was injected into cochlear basal ganglion to determine the amplitude of CM and Ap (N_1) before and after Ach treatment. The results showed that: 1, Ach solution (-4) M), the amplitude of Ap (N_1) was significantly decreased after injection of 0.3-1μl into the basilar drum. The amplitude of Ap (N_1) before Ach injection was 0.23 ± 0.05mv. The amplitude of Ap (N_1) The average value of 0.06 ± 0.03mv, Ap (N_1) amplitude decreased by an average of 83%. Experimental data by statistical t test, p <0.001. This result shows that Ach has a significant inhibitory effect on Ap (N_1). And latency of Ap (N_1) decreased by an average of 3.97 msec. This result was consistent with the previously reported inhibitory effects of electrical stimulation of cochlear efferent fibers on Ap (N_1) amplitude and prolonged latency. 2, Ach solution (0.5 × 10 ~ (-4) M) After the cochlea basal drum injection of 0.3-1μl, the CM amplitude was significantly reduced. The average amplitude of CM before Ach injection was 0.06 ± 0.01mv; the average value of CM amplitude after injection was 0.03 ± 0.01mv, and the amplitude of CM was reduced by 80% on average. p <0.001. The results showed that Ach significantly inhibited the amplitude of CM. As the amplitude of CM decreases, its latency is extended by an average of 0.49 msec. 3, observed in our work Ach on the inhibitory effect of cochlear potential, the performance of two modes of action, one is a sudden inhibition, inhibition of very fast, similar to Ach N role; the other is the table inhibition slow, long lasting , Similar to Ach’s M effect. Therefore, we hypothesize that it appears that the hair cells of the corti’s organ of the cochlea may also have two receptors for Ach. 4, according to previous work and our experimental study, it seems conceivable that the cochlear touch-tip inhibitory effect may be a cholinergic transmission mechanism.