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本文采用体内给药的方法，研究了雷公藤中抗生育有效化合物Ｔ４、Ｔ７在抗生育剂量下，对ＳＤ雄性大鼠生殖细胞染色体畸变率和微核形成的影响。大鼠口服Ｔ４抗生育剂量为每日８０μｇ／ｋｇ，Ｔ７为３１７μｇ／ｋｇ，１０周后制片分析，并与对照组比较，发现染色体畸变平均值各组之间的差异没有统计学意义（Ｐ＞０．０５），各组之间的生精细胞微核出现率也无统计学意义（Ｐ＞０．０５）。结果表明，雷公藤中抗生育有效成分Ｔ４、Ｔ７单体对大鼠精原细胞染色体的细胞遗传学效应和生精细胞微核发生率的影响结果是一致的，也提示抗生育剂量的Ｔ４和Ｔ７无明显的诱变作用。由于Ｔ７的各项指标更接近对照组，我们认为Ｔ７优于Ｔ４。
In this study, we investigated the effects of anti-fertility compounds T4 and T7 on the chromosome aberration rate and micronucleus formation of germ cells in male SD rats at different doses of anti-fertility. The oral dose of T4 was 80μg / kg and the T7 was 317μg / kg in oral administration. After 10 weeks of operation, the results of the analysis were compared with those of the control group. There was no significant difference between the groups in the mean of chromosome aberration (P > 0.05). The incidence of spermatogenic cell micronuclei in each group was also not statistically significant (P> 0.05). The results showed that the anti-fertility of Tripterygium T4, T7 monomer on rat spermatogonial chromosome cytogenetic effect and the incidence of spermatogenic cells micronucleus results are consistent, also prompted anti-fertility dose of T4 and T7 no obvious mutagenic effect. As the T7 indicators closer to the control group, we believe that T7 is better than T4.