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植物致有丝分裂因子如植物血凝素(PHA)、刀豆素A(ConA)、及美洲商陆(PWM),已常规用于刺激人淋巴细胞使之分裂。淋巴细胞的增殖反应,通常以摄取~3H-胸腺嘧啶掺入DNA来测定。在文献上,不同来源分离的人体T淋巴细胞,对上述致有丝分裂因子刺激的反应结果是一致的。另一方面,关于致有丝分裂因子诱发分离的人B淋巴细胞增殖也有许多不一致的报告。这些观察提示B淋巴细胞对植物致有丝分裂因子增殖反应的矛盾结果,最恰当的解释是B淋巴细胞分离方法的差别、有时是B淋巴细胞制备物的纯度不同。有人报告用E-玫瑰花方法分离的末梢血B淋巴细胞,对PHA、ConA、或PWM无反应,另一方
Plant-induced mitogenic agents such as phytohaemagglutinin (PHA), concanavalin A (ConA), and pokeweed (Pseudo-Americana) have conventionally been used to stimulate human lymphocytes to divide. The proliferative response of lymphocytes is usually determined by incorporation of ~ 3H-thymidine into DNA. In the literature, human T lymphocytes isolated from different sources are consistent in response to the mitogenic stimuli described above. On the other hand, there are also many inconsistent reports on the proliferation of human B lymphocytes induced by mitogenic factors. These observations suggest that the paradoxical result of B-lymphocyte proliferation responses to plant-induced mitogenic factors, the most appropriate explanation is the difference between B lymphocyte separation methods, and sometimes the purity of B-lymphocyte preparations. It has been reported that peripheral blood B lymphocytes isolated by the E-rose method do not respond to PHA, ConA, or PWM, and the other