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用电子捕获气相色谱(GC-ECD)、薄层色谱(TLC)和气相色谱-质谱联用(GC-MS)等方法对6个省区大骨节病区的玉米、小麦进行了镰刀菌毒素的检测和鉴定。结果表明,在4个省病区的玉米中均检测到脱氧雪腐镰刀菌烯醇(DON)、15-乙酰脱氧雪腐镰刀菌烯醇(15-ADON)、3-乙酰脱氧雪腐镰刀菌烯醇(3-ADON)、雪腐镰刀菌烯醇(NIV)和玉米赤霉烯酮(ZEA)等5种镰刀菌毒素;2个省病区的小麦中均检测到除15-乙酰脱氧雪腐镰刀菌烯醇(15-ADON)以外的其余4种镰刀菌毒素,病区与非病区之间,各毒素的污染水平呈显著差异;同省重轻病区之间,各毒素的污染率和平均污染水平存在差异,有的呈显著差异,玉米和小麦中均未检测到T-2毒素和镰刀菌氧萘满酮(TDP-1)等镰刀菌毒素。
The contents of Fusarium toxins in maize and wheat from 6 provinces and autonomous regions were analyzed by GC-ECD, TLC and GC-MS. Detection and identification. The results showed that DON, 15-ADON, 3-acetyl deoxynivalenol were detected in maize in four provinces. 5 kinds of Fusarium toxins such as 3-ADON, NIV and ZEA were detected in all the wheat in the two provincial wards except for 15-acetyl deoxy-dextrose Fusarium enolase (15-ADON), the remaining four kinds of Fusarium toxins, ward and non-ward, the levels of the toxins were significantly different levels of pollution; the province of light ward, the toxins contamination rate The mean level of pollution was different from that of the average level of pollution, and some of them were significantly different. Neither T-2 nor FDP (FDP) such as TDP-1 was detected in maize and wheat.