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报道了以２′，７′－二氯荧光素（ＤＣＦ）为指示剂，荧光法间接测定痕量酚的新方法。在０．５ｍｏｌ／ＬＨ２ＳＯ４介质中，Ｂｒ－和ＢｒＯ－３反应生成Ｂｒ２，Ｂｒ２与２′，７′－二氯荧光素反应，使２′７′－二氯荧光素荧光猝灭，当加入酚时，酚的溴代反应使体系荧光增强。ｐＨ４．０～６．０范围内，该体系激发波长，发射波长分别为λｅｘ５０５ｎｍ，λｅｍ５２０ｎｍ。酚浓度在１．６～５２ｎｇ／Ｌ范围内呈线性关系，检出限为１．６ｎｇ／Ｌ。本法选择性好，用于环境水样中酚的测定，结果满意。
A new method for the indirect determination of trace phenols by fluorescence was reported using 2 ’, 7’-dichlorofluorescein (DCF) as an indicator. In 0.5 mol / LH 2 SO 4 medium, Br 2 reacts with BrO 3 to form Br 2. Br 2 reacts with 2 ’, 7’ -dichlorofluorescein to quench the fluorescence of 2 ’7’ -dichlorofluorescein. When adding phenol When the phenolic bromine reaction makes the system fluorescence enhanced. pH4.0 ~ 6.0 range, the system excitation wavelength, emission wavelength were λex505nm, λem520nm. The concentration of phenol in the range of 1.6 ~ 52ng / L showed a linear relationship with a detection limit of 1.6ng / L. This method has good selectivity and is used for the determination of phenol in environmental water samples with satisfactory results.