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目的探讨抗生素及七味白术散对菌群失调腹泻小鼠乳糖酶基因13910位点多态性的影响。方法将36只SPF级小鼠随机分为正常组(12只)、模型组(12只)、七味白术散组(6只)和乳糖酶组(6只),除正常组外的其余小鼠采用混合抗生素造成菌群失调腹泻模型,然后分别灌胃七味白术散和乳糖酶治疗。造模成功和治疗后分别提取小鼠肠道内容物和肠道黏膜宏基因组,对包括小鼠乳糖酶基因13910位点在内的1 036bp长度的DNA片段进行单核苷酸多态性(SNP)测序分析。结果造模和治疗后,小鼠乳糖酶基因13910位点没有发生碱基的变异,均为A/A型,13910位点在内的1 036bp范围内也没有新的SNP位点出现。结论菌群失调腹泻造模与七味白术散治疗对小鼠乳糖酶活性的干预均与小鼠乳糖酶基因13910位点多态性没有相关性,可能存在其他多态性位点或其他方面的调控机制。
Objective To investigate the effect of antibiotics and Qiweibaizhu San on polymorphism of lactase gene 13910 in mice with dysbiligo diarrhea. Methods Thirty-six SPF mice were randomly divided into normal group (n = 12), model group (n = 12), Qiwei Baizhu Powder group (n = 6) and lactase group Mixed antibiotics caused by flora imbalance diarrhea model, and then respectively gavage with Baishan Baitai powder and lactase treatment. After the success of the model and the treatment, the gut contents of the intestinal mucosa and the gut mucosa were extracted respectively, and the single nucleotide polymorphism (SNP) of the 1 036 bp DNA fragment including the 13910 site of the mouse lactase gene ) Sequencing analysis. Results After modeling and treatment, there was no base mutation in the lactase gene 13910 of mouse. All of them were A / A type. There was no new SNP site in the range of 1 036 bp including 13910. Conclusion The results showed that there was no correlation between lactosease gene 13910 locus polymorphism and other polymorphic loci or other aspects in the treatment of mice with lactobacilli mechanism.