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小分子热激蛋白(sHsp)在植物抵抗非生物胁迫过程中起重要作用.采用生物信息学方法,在川桑(Morus notabilis基因组中鉴定了29个桑树sHsp基因,基于进化关系及亚细胞定位将其分为13个亚家族和1个孤儿基因.13个桑树sHsp基因亚家族中,CⅫ和CⅫ属于植物sHsp的2个新的亚家族;预测有4个桑树sHsp基因的启动子中各具1个完整热激元件(HSE),在桑树sHsp基因的启动子中可能还包含其他响应非生物胁迫的元件.采用定量PCR方法检测非生物胁迫下印度桑(Morus indcia)品种K2的叶片中10个桑树sHsp基因都能快速地响应高温胁迫,分别有5、9、7个桑树sHsp基因响应低温、盐分和干旱胁迫,其中Mn15.7-CV基因在4种逆境胁迫下的表达均被抑制,并在高温胁迫5 min后表达显著下调.研究结果提示sHsp可能在桑树抵抗非生物逆境胁迫的过程中发挥重要作用.“,”Small heat shock proteins (sHsps) play important roles in endurance of plants to abiotic stresses.A total of 29 genes encoding mulberry sHsps were identified from Morus notabilis genome by bioinformatic analysis.Based on evolutionary relationship and subcellular localization,these genes were classified into 13 subfamilies and an orphan gene.Among these 13 subfamilies,CXⅡ and CXⅢ belonged to two new subfamilies of plant sHsps.Except for one perfect heat shock element (HSE) found in the promoter of 4 mulberry sHsp genes,promoters of mulberry sHsp genes might contain some other elements involved in response to abiotic stresses.Quantitative PCR results indicated that 10 sHsp genes of mulberry (Morus indica) variety K2 were all able to respond to high temperature quickly.There were 5,9,and 7 mulberry sHsp genes showing response to low temperature,salt,and drought stress,respectively.The expression of Mn15.7-CV gene was repressed by all 4 kinds of environmental stresses and significantly down-regulated after 5 min under high temperature.These results suggest that mulberry sHsp genes play important roles in resisting abiotic stresses.