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【目的】将辐射诱变与离体选择相结合,筛选广藿香抗青枯病突变体,为广藿香抗病育种奠定基础。【方法】观察60Coγ射线辐射对广藿香离体再生的影响;以辐射诱变的广藿香为材料,以青枯菌粗毒素为选择压力,筛选抗青枯病植株。【结果】在进行抗性芽及抗性植株筛选中,分别以1.34×108cfu(菌落形成单位)/mL及2.48×108cfu/mL青枯菌制备的粗毒素作为选择压力,可能筛选到抗性的材料。60Coγ射线辐射对广藿香离体再生有明显的影响,随着辐射剂量的增加,外植体存活率及再生芽的能力显著下降。最终选取剂量为40 Gy和60 Gy进行辐射诱变,结合离体培养筛选抗病突变体,获得了24株抗性植株。【结论】建立了筛选广藿香抗病突变体的方法,为广藿香抗病育种提供了有价值的种质材料。
【Objective】 The objective of this study was to screen for the mutants of Patchouli resistance to bacterial wilt by combining mutagenesis and in vitro selection, which laid the foundation for the disease-resistant breeding of patchouli. 【Method】 The effects of 60Coγ-ray irradiation on the regeneration of patchouli in vitro were observed. The radiation-induced patchouli was used as the material and the strain of R. solanacearum was selected as the selection pressure to screen the plants resistant to the bacterial wilt. 【Result】 In the screening of resistant buds and resistant plants, the crude toxins prepared by 1.34 × 108 cfu / colony forming unit / mL and 2.48 × 108 cfu / mL R. solanacearum were selected as the selection pressure, material. 60Coγ-ray radiation had obvious effects on the regeneration of patchouli in vitro. With the increase of radiation dose, the survival rate of explants and the ability of regenerating shoots decreased significantly. Finally, the radiation doses of 40 Gy and 60 Gy were selected for the mutagenesis. The resistant mutants were screened in vitro and 24 resistant plants were obtained. 【Conclusion】 The method of screening the patchouli disease-resistant mutants was established, which provided valuable germplasm materials for the patchouli disease-resistant breeding.