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目的： 动态观察急性肺损伤（ ＡＬＩ） 大鼠肺组织白细胞介素－ １３（ＩＬ－ １３） ｍ ＲＮＡ 表达的变化，初步探讨ＩＬ－ １３ ｍ ＲＮＡ 的变化与ＡＬＩ 发生、发展的关系。方法： 在脂多糖诱发的ＡＬＩ 大鼠模型，采用ＲＴ－ ＰＣＲ 方法，检测肺组织ＩＬ－ １３ ｍ ＲＮＡ 的表达。结果：正常肺组织表达一定水平的ＩＬ－ １３ ｍ ＲＮＡ；在ＡＬＩ 大鼠肺组织，ＩＬ－ １３ ｍ ＲＮＡ 表达呈短暂性上调，随即逐渐下调。结论： 正常肺组织可表达一定水平的ＩＬ－ １３ ｍ ＲＮＡ；在ＡＬＩ肺组织，ＩＬ－ １３ ｍ ＲＮＡ 表达逐渐下调，提示局部抗炎作用减弱，可能引起抗炎和致炎作用失衡，从而促进急性呼吸窘迫综合征的发生。
OBJECTIVE: To dynamically observe the expression of interleukin-13 (IL-13) m RNA in lung of acute lung injury (ALI) rats and to explore the relationship between the changes of IL-13 mRNA and the occurrence and development of ALI. Methods: The expression of IL-13 mRNA in lung tissue was detected by RT-PCR in lipopolysaccharide-induced ALI rat model. Results: IL-13 mRNA was expressed in normal lung tissue. IL-13 mRNA expression was transiently up-regulated in lung tissue of ALI rats, and then decreased gradually. CONCLUSION: The normal lung tissue can express a certain level of IL-13 m RNA. In ALI lung tissue, the expression of IL-13 m RNA gradually decreases, suggesting that local anti-inflammatory effect may be weakened, which may lead to imbalance of anti-inflammatory and proinflammatory effects and promote acute Respiratory distress syndrome.