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对沈阳、河北、吉林、北京等地区玉米穗、茎腐病禾谷镰孢菌和串珠镰孢菌进行了可溶性蛋白和同工酶聚丙烯酰胺凝胶电泳,以研究玉米穗、茎腐病同种镰孢菌是否存在寄生专化性或生理分化现象。结果表明:各地区的玉米穗、茎腐病串珠镰孢菌同源性高,亲缘关系近,相似性系数在0.86~0.98之间,菌株间差异仅表现为个体间多样性。而两种病害禾谷镰孢菌亲缘关系困地区不同而有差异,河北、吉林两地区穗、茎腐禾谷镰孢菌间均具有很近的亲缘关系,相似性系数在0.93以上;但沈阳地区禾谷镰孢菌亲缘关系相对较远,相似性系数仅为0.65~0.67。
Soluble protein and isoenzyme polyacrylamide gel electrophoresis were carried out on the ear of corn, stalk rot of Fusarium graminearum and Fusarium moniliforme in Shenyang, Hebei, Jilin, Beijing and other regions to study the relationship between ear rot and stalk rot Fusarium species exist parasitic or physiological differentiation phenomenon. The results showed that the homology of Fusarium moniliforme in maize ear and stalk rot was high in all regions, and the genetic similarity was between 0.86 and 0.98. The difference among isolates showed only inter-individual diversity. However, the genetic relationship between the two diseases and Fusarium graminearum was different. The genetic relationship between Fusarium graminearum and Fusarium graminearum in Hebei and Jilin was close, with a similarity coefficient of 0.93 or above. However, the genetic relationship between Fusarium graminearum in Shenyang was relatively far, and the similarity coefficient was only 0.65 ~ 0.67.