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目的评价常用淋病双球菌实验室诊断方法的临床实用性。方法疑诊淋病双球菌感染患者186例,分别采用直接涂片染色法、分离培养法和实时荧光定量聚合酶链反应法(real time fluorescent quantitative-polymerase chain reaction,FQ-PCR)检测男性尿道分泌物、女性宫颈分泌物标本,并比较急性和慢性感染者、男性和女性感染者3种检测方法的阳性率。结果直接涂片染色法、分离培养法和FQ-PCR法检测淋病双球菌的阳性率分别为51.08%,54.30%,62.90%;慢性感染者、男性感染者FQ-PCR检测阳性率均高于直接涂片染色法、分离培养法(P<0.05);急性感染者、女性感染者3种检验方法阳性率比较差异均无统计学意义(P>0.05)。结论临床应根据不同类型的淋病双球菌感染选择恰当的检测方法。
Objective To evaluate the clinical utility of commonly used gonorrhea gonococcal laboratory diagnostic methods. Methods Totally 186 patients with suspected Gonorrhea infection were tested for direct urethral discharge (MUR) using direct smear staining, isolation and culture, and real-time fluorescence quantitative-polymerase chain reaction (FQ-PCR) , Female cervical secretions samples, and compared the positive rate of the three detection methods of acute and chronic infections, male and female patients infected. Results The positive rates of FQ-PCR detection by direct smear staining, isolation and culture and FQ-PCR were 51.08%, 54.30% and 62.90% respectively. The positive rate of FQ-PCR in chronic infection and male infection was higher than that of direct (P <0.05). There was no significant difference in the positive rates of the three methods between acute infection and female infection (P> 0.05). Conclusion Clinic should choose appropriate detection method according to different types of gonorrhea.