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以前的研究将水稻光敏核不育位点Pms1定位在第7染色体上.本研究对来源于定位亲本(明恢63和农垦58)的两个BAC克隆进行比较测序,以鉴定Pms1的候选基因;并通过对两个克隆的注释和比较得到5个候选基因,以进行进一步的功能检验.将这两个亲本的基因组序列与公共数据库里的日本晴和93-11比较分析,结果显示,该区段4个亲本在序列组成上存在巨大差异,这种差异主要归咎于活性反转座成分造成的基因组新近增长和变异;亚种内和亚种间以Indel或者SNP的形式存在高度的多态性.利用反转座成分的两个长末端重复进行分析发现,它们的替换速率比已有的报道要高得多.该结果证明,在自然和人工选择的共同作用下,Pms1区段正处于快速的基因组进化过程中.
Previous studies have mapped Pms1, a photoperiod-sensitive genic male sterile site on chromosome 7. Two BAC clones derived from the mapped parents (Minghui63 and Nongken58) were compared and sequenced to identify candidate genes for Pms1. And five candidate genes were obtained by annotation and comparison of the two clones for further functional tests.The genome sequences of the two parents were compared with Nipponbare and 93-11 in a public database and the results showed that the segment The four parents had a huge difference in the sequence composition. The difference was mainly attributed to the recent increase and variation of the genome caused by the active retrofix composition. There was a high degree of polymorphism in the subspecies Indel or SNP. Repeated analysis using the two long ends of the inverted pedestal revealed that they were replaced at a much higher rate than previously reported, demonstrating that the Pms1 segment is rapidly undergoing natural and artificial selection Genome evolution.