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首次将胶体金作为标记物引入相变免疫分析中 ,建立了吗啡免疫分析的新方法。该分析方法是将吗啡抗体与温度敏感型水凝胶偶联 ,胶体金标记吗啡全抗原 ,再进行相变分离 ,最后用可见吸收光谱仪进行检测。本方法的两个重要特点是 :( 1)利用温敏水凝胶的相变特性进行均相反应和异相分离 ,缩短了免疫反应时间 ,简化了步骤 ;( 2 )利用胶体金快速稳定吸附蛋白质的特性进行标记 ,利用胶体金本身的颜色 ,可进行定性检测 ,或者用常规的可见吸收光谱仪进行定量检测 ,对仪器要求简单 ,适用于临床检测的要求。
For the first time, colloidal gold was used as a marker in the phase-change immunoassay and a new method of morphine immunoassay was established. The method is to couple morphine antibody with temperature-sensitive hydrogel, colloidal gold labeled with morphine antigen, and then phase separation, and finally with visible absorption spectroscopy. Two important features of this method are: (1) homogeneous and heterogeneous phase separation using the phase-change characteristics of a thermosensitive hydrogel, shortening the immunoreaction time and simplifying the steps; (2) rapid and stable adsorption of protein using colloidal gold Characteristics of the mark, the use of colloidal gold itself, the color can be qualitative detection, or conventional visible absorption spectroscopy for quantitative detection of the instrument requires a simple, suitable for clinical testing requirements.