Expression of Helicobacter pylori Hsp60 protein and its immunogenicity

• 作 者: YangBai Liang-RenLi Ji-DeWang
• 机 构: PLAInstituteforDigestiveMedicine,ChemistryUniversityofBeijing,InstituteofBiotechnology
• 出 处: 世界胃肠病学杂志：英文版（电子版）
• 发表日期: 2003年12期
• 关键词: 热休克蛋白60 幽门螺杆菌 免疫原性 PCR DNA序列分析
• 基金项目: 国家自然科学基金

AIM: To express Hsp60 protein of H pylori by a constructed vector and to evaluate its immunogenicity.METHODS: Hsp60 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+), which was transformed into BL21 (DE3) E. coli str