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目的 探讨67ku层粘连蛋白受体(67LR)基因表达与子宫内膜癌的关系。方法 应用免疫组化SABC法检测1990～2001年收治的44例子宫内膜癌、15例子宫内膜增殖症、47例正常子宫内膜组织中67LR的表达;采用荧光定量逆转录聚合酶链反应(FQ-RT-PCR)方法定量检测2000～2001年收治的24例子宫内膜癌、16例子宫内膜增殖症、29例正常子宫内膜新鲜组织67LRmRNA水平。结果 67LR在子宫内膜癌、子宫内膜增殖症的表达较正常子宫内膜均明显增高(P<0.001),但前两组的表达差异无显著性(P>0.05)。高、中分化子宫内膜腺癌67LR表达较低分化者为高(P<0.05);但高、中、低分化组病例67LR mRNA水平差异无显著性(P>0.05)。67LR mRNA水平与免疫组化蛋白表达无明显相关(P>0.05)。结论 67LR蛋白过度表达可能与子宫内膜癌的发生、发展有关。67LR mRNA水平与蛋白表达水平无相关。
Objective To investigate the relationship between 67ku laminin receptor (67LR) gene expression and endometrial carcinoma. Methods Immunohistochemical SABC method was used to detect the expression of 67LR in 44 cases of endometrial carcinoma, 15 cases of endometrial hyperplasia and 47 cases of normal endometrium from 1990 to 2001. The expression of 67LR was detected by fluorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) was used to detect the levels of 67LR mRNA in 24 cases of endometrial cancer, 16 cases of endometrial hyperplasia and 29 cases of normal endometrial freshmen admitted from 2000 to 2001. Results The expression of 67LR in endometrial carcinoma and endometrial hyperplasia was significantly higher than that in normal endometrium (P <0.001). However, there was no significant difference between the two groups (P> 0.05). The levels of 67LR mRNA in high and moderately differentiated endometrial adenocarcinomas were higher than those in lower differentiated endometrial adenocarcinomas (P <0.05). However, there was no significant difference in 67LR mRNA levels between high and moderately differentiated endometrial adenocarcinomas (P> 0.05). There was no significant correlation between 67LR mRNA and immunohistochemistry (P> 0.05). Conclusion 67LR protein overexpression may be related to the occurrence and development of endometrial cancer. There was no correlation between 67LR mRNA and protein expression.