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植物名称:长寿花(Narcissus jonquilla)。材料类别:茎尖和带腋芽茎段。培养条件:嫩茎用自来水洗净后,用70％酒精进行表面消毒30秒钟,再用0.1％升汞溶液消毒8分钟,然后用无菌水洗4次,在无菌条件下将嫩茎切成1cm左右的茎尖和带1～2个节的茎段。以MS为基本培养基,诱导分化和生长培养基为MS+BA1.0mg/L(单位下同)+NAA0.1,生根培养基为1/2MS+NAA0.1～1.0。3％蔗糖可用白糖代替,温度为25±2℃,每天光照10小时,光照强度1500～2000lx。
Plant name: longevity flower (Narcissus jonquilla). Material category: shoot tips and stems with axillary buds. Culture conditions: tender stems washed with tap water, surface sterilized with 70% alcohol for 30 seconds and then disinfected with 0.1% mercuric chloride solution for 8 minutes, and then washed with sterile water 4 times under aseptic conditions will be cut stems Stem tips into about 1cm and stems with 1 to 2 sections. With MS as the basic medium, induced differentiation and growth medium for the MS + BA1.0mg / L (the same below) + NAA0.1, rooting medium for 1 / 2MS + NAA0.1 ~ 1.0.3% sucrose available sugar Instead, the temperature of 25 ± 2 ℃, light per day 10 hours, light intensity 1500 ~ 2000lx.