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目的 探讨在非霍奇金淋巴瘤中p16蛋白和增殖细胞核抗原 (PCNA)的表达情况及相关性意义。方法 应用免疫组化LSAB法检测 41例NHLp16和PCNA表达情况 ,并作相关性分析。结果 41例NHL中 ,p16蛋白阳性表达率 43 9% ( 18/ 4 1) ,PCNA阳性表达率 65 9% ( 2 7/ 4 1)。p16蛋白在低度恶性组表达率 77 8% ,在高度恶性组表达率 17 4% (P <0 0 1)。PCNA在低度恶性组表达率44 4% ,高度恶性组表达率 82 6% (P <0 0 5 )。p16蛋白表达阴性同时PCNA表达阳性者在低度恶性组 ( 5 6% )与高度恶性组( 65 2 % )之间有显著性差异 (P <0 0 1)。结论 在NHL发展中 ,p16蛋白缺失表达起着重要作用 ,而结合p16蛋白和PCNA在NHL的表达结果 ,对推测NHL的恶性程度和预后有重要意义。
Objective To investigate the expression and significance of p16 protein and proliferating cell nuclear antigen (PCNA) in non-Hodgkin’s lymphoma. Methods The expression of NHLp16 and PCNA in 41 cases were detected by immunohistochemical LSAB method, and the correlation analysis was performed. Results The positive expression rate of p16 protein was 43 9% (18/41) in 41 cases of NHL, and the positive rate of PCNA was 65 9% (27/41). The expression rate of p16 protein was 77.8% in the low-grade group and 174% in the high-grade group (P < 0 01). The expression rate of PCNA in the low-grade group was 44%, and the high-grade group was 82.6% (P < 0.05). There was a significant difference between the low-grade (56%) and high-grade (65%) groups in patients with negative p16 protein expression and positive PCNA expression (P < 0.01). Conclusions The deletion of p16 protein plays an important role in the development of NHL. The expression of p16 protein and PCNA in NHL is of great significance for estimating the malignancy and prognosis of NHL.