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植物在感知到虫害信号后会经过复杂的转录调控,开启防御基因的表达。这一过程大多受到茉莉酸信号途径的调控,现有的相关研究主要集中在双子叶模式植物拟南芥中,在单子叶植物玉米中是否具有相似的转录调控模式目前尚不清楚。为了探索这一问题,从被玉米螟咬食和150μmol/L JA处理12 h的RNAseq玉米文库中获得两个同时受诱导的基因,Zm281和Zm Pal,重点对其启动子的诱导活性进行了分析。结果表明,这两个玉米启动子在未受JA处理的拟南芥中不能启动GUS报告基因的表达,但是在茉莉酸诱导后GUS报告基因受到强烈诱导,且在各组织器官中均有表达。该结果表明受虫咬后诱导表达的基因同时受JA的诱导,可为研究JA诱导虫害应答的分子机理奠定基础。
After the plant detects the pest signal, it undergoes complicated transcriptional regulation and turns on the defense gene expression. Much of this process is regulated by the jasmonate signaling pathway. The current research focuses on the dicotyledonous plant Arabidopsis thaliana, and whether or not there is a similar pattern of transcriptional regulation in monocotyledonous corn is not clear. In order to explore this problem, two simultaneously induced genes, Zm281 and Zm Pal, were obtained from RNAseq maize libraries that were inoculated with corn borer and treated with 150μmol / L JA for 12 h, and the inducing activity of their promoters was analyzed . The results showed that these two maize promoters could not start GUS reporter gene expression in JA-treated Arabidopsis, but GUS reporter gene was strongly induced after jasmonic acid induction and expressed in all tissues and organs. The results indicated that the genes induced by insect bites were induced by JA at the same time, which laid the foundation for studying the molecular mechanism of JA-induced pest responses.