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目的 研究大鼠服药后胆汁中盐酸非洛普 (DDPH)I相代谢物。方法 大鼠做胆管插管 ,分别收集ipDDPH之前的空白胆汁及服药后 12h内的服药胆汁 ,将大鼠胆汁以葡糖醛酸酶水解后进C 18SPE小柱进行纯化富集 ,再进行LC/DAD/MSD分析 ;同时将合成的 6个DDPH模拟代谢物M1-M6的对照品混合液按相同条件进行LC/DAD/MSD分析对照。结果 大鼠服药胆汁色谱图中峰A ,B ,C ,D ,E和F分别与M1,M2 ,M3,M5,M4 和M6的保留时间、紫外吸收光谱、分子量及碎片离子完全一致。结论 M1,M2 ,M3,M4 ,M5和M6为大鼠ipDDPH后产生的体内I相代谢物
Objective To study metabolites of phase I metabolite of felodipine hydrochloride (DDPH) in rat bile. Methods The rats were given bile duct intubation, and the blank bile before ipDDPH and the bile within 12h after taking the drug were collected respectively. The rat bile was hydrolyzed with glucuronidase and then purified by C 18 SPE cartridge. LC / DAD / MSD analysis. At the same time, LC-DAD / MSD analysis of 6 synthetic DDPH analog metabolites M1-M6 reference standard mixture under the same conditions. Results The peak A, B, C, D, E and F in the rat bile chromatogram were completely consistent with the retention times, UV absorption spectra, molecular weights and fragment ions of M1, M2, M3, M5, M4 and M6, respectively. Conclusions M1, M2, M3, M4, M5 and M6 are the in vivo phase I metabolites produced by ipDDPH in rats