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人脐带组织富含间充质干细胞(human umbilical cord mesenchymal stem cells,hUCMSCs),是干细胞研究理想的种子来源,如何从脐带组织中分离间充质干细胞及运用影像技术示踪干细胞生物学行为是当前研究的热点。该实验应用组织块贴壁法从足月孕妇脐带组织中分离纯化间充质干细胞并进行鉴定,结果为hUCMSCs。进一步应用磷酸钙、Effectene、脂质体2000三种转染试剂分别介导Gd-DTPA标记hUCMSCs,通过MRI(magnetic resonance imaging)检测钆喷酸葡胺(Gd-DTPA)标记细胞信号强度变化及细胞内钆离子浓度的测定评价三种转染试剂的转染能力,最终发现在三种转染试剂中,Effectene介导Gd-DTPA标记hUCMSCs效果最佳,为Gd-DTPA标记干细胞体外MR成像奠定了实验基础。
Human umbilical cord tissue is rich in human umbilical cord mesenchymal stem cells (hUCMSCs), is an ideal source of stem cells for seed research, how to separate mesenchymal stem cells from umbilical cord tissue and using imaging techniques to track the biological behavior of stem cells is the current Hot research. In this experiment, mesenchymal stem cells were isolated and purified from umbilical cord tissues of full-term pregnant women using tissue block adhesion method and identified as hUCMSCs. Further, calcium phosphate, Effectene and lipofectamine 2000 were respectively used to mediate Gd-DTPA labeled hUCMSCs. The signal intensity of Gd-DTPA labeled cells was detected by MRI (magnetic resonance imaging) Gd-DTPA labeled hUCMSCs mediated by Effectene were found to be the best among the three transfection reagents, which laid the foundation for in vitro MR imaging of Gd-DTPA-labeled stem cells Experimental basis.