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目的:探讨高原地区健康人群中抗核抗体(ANA)、抗胃壁细胞抗体(PCA)、抗平滑肌抗体(SMA)、抗线粒体抗体(AMA)、抗双链DNA抗体等自身抗体的阳性分布趋势,及其在疾病中的诊断价值。方法:用间接免疫荧光法检测1 275名健康人群血清标本中ANA、PCA、SMA、AMA和抗dsDNA抗体,用免疫印迹法检测ANA抗体谱。结果:ANA、PCA、SMA、AMA在1 275名健康人群血清标本中的阳性率分别为:22.90%、0.31%、2.66%、1.10%,抗ds-DNA抗体全部阴性。ANA抗体谱阳性率为2.90%,其中有6例ANA抗体谱阳性但ANA荧光阴性,262例ANA荧光检测阳性但ANA抗体谱未检测出相应靶抗原。女性ANA抗体谱阳性率显著高于男性(P<0.01),ANA、SMA、PCA、AMA阳性率在男女性别的差异无统计学意义(P>0.05)。ANA在人群中阳性率随年龄增加而升高(P<0.01),而SMA、PCA、AMA、ANA抗体谱在不同年龄组之间阳性率无差异(P>0.05)。ANA、PCA、SMA、AMA在1 275名健康人群血清标本中<320的滴度分别占81.85%、75%、79.41%、85.71%。结论:自身抗体的存在并不意味着自身免疫性疾病,健康人群中自身抗体有一定的阳性率,但是滴度较低,一些自身抗体如抗ds-DNA抗体可以作为某种自身免疫病的标志性抗体。应重视自身抗体的检测,间接免疫荧光法和免疫印迹法平行检测可以提高检出率,以防漏检。
Objective: To investigate the positive distribution trend of anti-nuclear antibody (ANA), anti-parietal cell antibody (PCA), anti-smooth muscle antibody (SMA), anti-mitochondrial antibody (AMA) And its diagnostic value in the disease. Methods: ANA, PCA, SMA, AMA and anti-dsDNA antibody were detected by indirect immunofluorescence in serum samples of 1 275 healthy people. The antibody spectrum of ANA was detected by immunoblotting. Results: The positive rates of ANA, PCA, SMA and AMA in serum samples of 1 275 healthy people were 22.90%, 0.31%, 2.66% and 1.10%, respectively. The anti-dsDNA antibodies were all negative. The positive rate of ANA antibody spectrum was 2.90%, of which 6 were positive for ANA antibody but negative for ANA fluorescence, while 262 for ANA fluorescence were positive but the corresponding target antigen was not detected for ANA antibody. The positive rate of ANA antibody in women was significantly higher than that in men (P <0.01). The positive rates of ANA, SMA, PCA and AMA in men and women were not statistically different (P> 0.05). The positive rate of ANA in the population increased with age (P <0.01), while the positive rates of SMA, PCA, AMA and ANA in different age groups showed no difference (P> 0.05). The titers of <320 in serum samples of ANA, PCA, SMA and AMA were 81.85%, 75%, 79.41% and 85.71% respectively in 1 275 healthy people. CONCLUSIONS: The presence of autoantibodies does not imply autoimmune disease. Autoantibodies in healthy individuals have a positive rate, but the titre is low. Some autoantibodies such as anti-dsDNA antibodies may serve as markers for some autoimmune diseases Sexual antibodies. Should attach importance to the detection of autoantibodies, indirect immunofluorescence and Western blot parallel detection can improve the detection rate to prevent missed.