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以牙鲆(Paralichthys olivaceus)为母本,圆斑星鲽(Verasper variegatus)为父本进行科间远缘杂交,利用ISSR标记对2个家系杂交子代进行扩增,分析了双亲位点在杂交子代中的传递分离方式以及双亲与杂交子代的遗传距离。结果表明:6个引物在0401~#和0402~#2个家系中扩增的位点数为136和144个,其中多态性住点数量为90和91个,分别占66.18%和63.19%,2个家系间没有明显差异。2个家系中双亲与杂交子代的共有位点数量分别达34.56%和38.89%,表明遗传物质传递给了杂交子代,证明了科间远缘杂交的成功。2个家系中符合孟德尔分离规律的位点分别占32.35%和22.92%,出现分离但偏孟德尔分离位点占23.53%和27.78%,2个家系中均观察到部分非亲位点。在这2个家系中,杂交子代与母本牙鲆的遗传距离分别为0.2941、0.3182,而与父本星鲽的遗传距离分别为0.3645、0.3750,表明杂交子代在遗传上偏向母本牙鲆。该研究结果为进一步利用杂交群体同时构建牙鲆和星鲽的遗传连锁图谱提供了重要参考。
Paralichthys olivaceus was used as female parent and Verasper variegatus as male parent for interspecific distant crosses. The ISSR markers were used to amplify the two offspring of hybrids. Separation of transmission in offspring and the genetic distance between parents and offspring. The results showed that the number of loci amplified by 6 primers in 0401 ~ # and 0402 ~ # 2 families was 136 and 144, and the number of polymorphic loci was 90 and 91, accounting for 66.18% and 63.19% respectively, There was no significant difference between the two families. The total number of shared sites between parents and hybrid progeny in the two families was 34.56% and 38.89%, respectively, indicating that the genetic material was passed to the hybrid progeny and the success of crossbreeding between families was proved. Among the two families, Mendel’s law of separation accounted for 32.35% and 22.92%, respectively, but there were 23.53% and 27.78% of the separated Mennonite and some non-parents were observed in the two families. Among the two pedigrees, the genetic distances between hybrid offspring and the parental flounder were 0.2941 and 0.3282, respectively, while the genetic distances to the male parent starlings were 0.3645 and 0.3750, respectively, indicating that the hybrid offspring were genetically biased towards the maternal teeth Flounder The results of this study provide important reference for further utilizing the hybrid population to construct the genetic linkage map of Japanese flounder and Halibut.