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采用改良Solt-Faber法建立大鼠肝癌前病变模型,以大豆异黄酮和皂甙饲喂大鼠42 d后,ELISA法测定血清肿瘤坏死因子-α(TNF-α),分光光度法测定γ-谷酰胺转肽酶(γ-GT)、丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)和一氧化氮(NO)含量。结果表明:大豆异黄酮和皂甙降低肝癌前病变大鼠血清γ-GT、ALT、AST活性,升高血清SOD、CAT、GSH-PX活性和降低MDA以及NO水平,但对血清TNF-α水平没有显著影响。表明大豆异黄酮和皂甙具有明显的抗化学致癌作用,其作用机制可能与增高抗氧化活性有关。
The model of precancerous lesions of rat hepatocarcinoma was established by modified Solt-Faber method. The rats were fed with soy isoflavones and saponins for 42 days. Serum tumor necrosis factor-α (TNF-α) was measured by ELISA and γ-ray was determined by spectrophotometry (Γ-GT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), catalase GSH-PX activity and malondialdehyde (MDA) and nitric oxide (NO) contents. The results showed that soy isoflavones and saponins could reduce the serum levels of γ-GT, ALT and AST, increase the activity of SOD, CAT, GSH-PX and decrease the levels of MDA and NO in serum of rats with precancerous lesions, but not for serum TNF- Significantly affected. These results indicate that soy isoflavones and saponins have obvious anti-carcinogenic effects and their mechanism may be related to the increase of anti-oxidative activity.