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目的：优选壮药矮陀陀中总生物碱的含量测定条件，并对不同产地药材进行含量比较。方法：采用正交试验法，以总生物碱含量为评价指标，以溶剂用量、超声时间、超声提取次数、缓冲液pH为考察因素，优选壮药矮陀陀中总生物碱含量测定的条件。采用优选的含量测定条件，测定18个产地不同采收季节药材的总生物碱含量。结果：优选的含量测定条件为溶剂用量（三氯甲烷）20 ml，超声处理3次，每次超声15 min，缓冲液pH为4.5。18个产地药材总生物碱含量在0.6～11.98 mg/g之间，含量差异较大。其中以隆林县、田林县在10月份采收的药材总生物碱含量最低。结论：优选的含量测定条件可用于测定壮药矮陀陀中总生物碱的含量及该药材的质量控制。该药材生物碱含量与产地、采收季节等有关。“,”OBJECTIVE:To optimize the content determination conditions of total alkaloids from Zhuang medicine Munronia delavayi,and to determine the content of total alkaloids in M. delavayi from different producing areas. METHODS:With the con-tent of total alkaloids as index,using solvent amount,ultrasonic time,ultrasonic extraction times and pH value of buffer as fac-tors,the content determination conditions of total alkaloids from Zhuang medicine M. delavayi were optimized by orthogonal test. Optimized content determination conditions were adopted to determine the content of total alkaloids in M. delavayi from 18 produc-ing areas in different harvest time. RESULTS:The optimum content determination conditions were as follows as the amount of sol-vent(CHCl3)20 ml,ultrasonic processing for 3 times,lasting for 15 min each time,pH value of buffer 4.5. The contents of total alkaloids in M. delavayi from 18 producing areas were between 0.6-11.98 mg/g,showing great difference. M. delavayi from Long-lin county and Tianlin county harvested in Oct. had the lowest content of total alkaloids. CONCLUSIONS:Optimized content deter-mination condition can be used for the content determination of total alkaloids in Zhuang medicine M. delavayi and quality control of it. The content determination of total alkaloids in M. delavayi is related to producing area and harvest time.